Laboratory on DNA Manipulation

Handouts

Protocols

  1. Quikchange site-directed kit

Powerpoint Slides

  1. Slides
  2. Slides
  3. Slides
  4. Slides
  5. Slides
  6. Slides
  7. Slides

Readings, not required but cited in class

References for papers

  1. MMBR Kranz et al 2009
  2. 2010_Young (Schultz) (Review - unnatural amino acids)
  3. 2013_Lajoei (Isaacs) (unnatural amino acids)
  4. Plant transformation review
  5. Promoter review
  6. Corn hybrid summary
  7. Link to the gene constructs in the VT Triple Pro

Quicktime Movies

  1. Dye Terminator Sequencing Reaction Mixture


Lab Websites


Natural Sciences Learning Center
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